Coding

Part:BBa_K5466010:Design

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-23)


LexA-VP16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 300
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The LexA DNA-binding domain (DBD), from Escherichia coli. The VP16 activation domain, from herpes simplex virus.

SV40 NLS was attached to the C-terminal.

S. cerevisiae codon optimized

Source

From iGEM20_UCopenhagen BBa_K3617001.